reverse phase hplc造句
例句與造句
- The method for the determination of amino acid neurotransmitters in brain by reverse phase hplc system with the precolumn phthali aldehyde derivatization
柱前衍生反相高效液相色譜法測(cè)定腦組織中氨基酸類(lèi)神經(jīng)遞質(zhì) - It is introduced that the quantitative analysis of trichlorfon by reverse phase hplc , using acetonitrile and water ( 50 : 50 ) as mobile phase , using cn column and ultraviolet detector
摘要本文采用即以乙腈+水( 50 + 50 )為流動(dòng)相，用cn柱和紫外檢測(cè)器分離測(cè)定敵百蟲(chóng)原藥及40 %敵?毒乳油中敵百蟲(chóng)反相高效液相色譜法。 - The quantitative analysis of 25 % chlorpyrifos and triazophos ec by reverse phase hplc was introduced , using acetonitrile and water ( 74 : 26 ) as mobile phase , using c18 column and ultraviolt detector
摘要本文敘述了采用反相高效液相色譜法即以乙腈+水為流動(dòng)相， c18柱和紫外檢測(cè)器同柱分離側(cè)定25 %毒?唑乳油中的毒死蜱和三唑磷。 - Imidacloprid and triazophos in mixture were determined simultareously by reverse phase hplc , methanol and acetonitrile and water and glacial acetic acid as mobile phase , c18 column and uv200 detecter
摘要采用c18不銹鋼柱和uv200型檢測(cè)器，以甲醇?乙腈?水的混合液為流動(dòng)相，用反相高效液相色譜法對(duì)吡蟲(chóng)啉?三唑磷復(fù)配制劑進(jìn)行定量分析測(cè)定。 - The crude venom contains a mixture of compounds with different types of biological activities . two novel peptide neueotoxins were purified from the venom of chilobrachys jingzhao by using combination of ion - exchange and reverse phase hplc
海南敬釗纓毛蛛( chilobrachysjingzhao )毒素是最近在海南發(fā)現(xiàn)的蜘蛛新種，該蜘蛛粗毒中含有多種活性多肽和蛋白質(zhì)成分。 - It's difficult to find reverse phase hplc in a sentence. 用reverse phase hplc造句挺難的
- Secondly , determination of the antimicrobial activities of synthetic hpab - . i ) the mature peptide of hpab was synthesized by fomc method and purified by reverse phase hplc . the molecular weight of synthesis peptide was 4334 . 96da characterized by mass spectrometry , highly agreed with the theoretical mw of hpab - 3
2 . hpab一p抗菌活性初步鑒定:采用固相法合成了hpab一p成熟膚，經(jīng)即一hplc純化后用質(zhì)譜測(cè)定合成膚的分子量為4334 . % da ，與其理論分子量相符。 - Both hainantoxin - iv ( hntx - iv ) and . hainantoxin - v ( hntx - v ) w ere peptide toxins isolated from the venom of the spider seleconosmia hainana by means of ion - exchange chromatograph and reverse phase hplc . hntx - iv , 3989 da , contained 35 amino acid residues with three disulfide bonds , while the molecular weight of hntx - v was 3972 da and had three disulfide bonds , too
海南捕鳥(niǎo)蛛毒素- ( hntx - )和海南捕鳥(niǎo)蛛毒素- ( hntx - )是從海南捕鳥(niǎo)蛛粗毒中經(jīng)陽(yáng)離子交換和反相hplc柱層析分離出來(lái)的兩種神經(jīng)毒素， maldi - tof質(zhì)譜鑒定前者相對(duì)分子質(zhì)量為3 ， 989da ，含有三對(duì)二硫鍵，由35個(gè)氨基酸殘基組成；后者相對(duì)分子質(zhì)量為3 ， 972da ，與前者只相差16個(gè)質(zhì)子，也含有三對(duì)二硫鍵。 - The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經(jīng)blgii線(xiàn)性化后，轉(zhuǎn)化酵母宿主菌gs115原生質(zhì)體后經(jīng)篩選陽(yáng)性克隆并經(jīng)表型鑒定為his ~ + mut ~ s酵母菌，進(jìn)一步用遺傳毒素g418篩選多拷貝的轉(zhuǎn)化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導(dǎo)表達(dá)，發(fā)酵上清經(jīng)90飽和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超濾膜超濾，再經(jīng)cm陽(yáng)離子交換， c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經(jīng)質(zhì)譜鑒定，氨基酸組成分析和序列測(cè)定為正確的表達(dá)產(chǎn)物，生物學(xué)活性表明其活性為天然毒素活性70 % ，表達(dá)量為80mg / l 。 - A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc . hwtx - v has 35 amino acid residues , with the molecular mass 4111 . 4da . the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh , which consists of 6 cys , formed three pairs of disulfide bridges
本文報(bào)道從虎紋捕鳥(niǎo)蛛( selenocoimahuwena )粗毒中，結(jié)合陽(yáng)離子交換和反相高效液相色譜分離純化出一種昆蟲(chóng)毒素，命名為虎紋捕鳥(niǎo)蛛毒素- ( huwentoxin - ， hwtx - ) ；其天然突變體( themutantofhuwentoxin ， mhwtx - )也同時(shí)純化出來(lái)。